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US 4,666,832 AGranted
Pyruvate oxidase
AssigneeBoehringer Mannheim GmbH
InventorsErich Elstner, Karl-Heinz Schleifer, Friedrich Gotz, Barbara Sedewitz, Albert Roder, Hans Mollering, Hans Seidel
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Abstract
The present invention provides a new pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, characterized in that it is active without the addition of FAD, TPP and divalent metal ions. The present invention also provides a process for preparing this new enzyme and a reagent containing it.
Claims10
A purified pyruvate oxidase which decarboxylates pyruvate with the formation of hydrogen peroxide, is active without the addition of FAD, TPP and divalent metal ions and has an activity optimum at pH 5.7.
Dependencies are inferred from the claim text.