Production of reverse transcriptase
AssigneeMolecular Genetics, Inc.
InventorsJohn H. Weis, John S. Salstrom
Life of this patent
Estimating the term…
Abstract
Methods are described for the production of the .beta.-subunit of reverse transcriptase using recombinant DNA techniques. Methods and compositions are described for cloning and expressing the gene coding for the .beta.-subunit of reverse transcriptase and characterizing and isolating the gene product. The recombinant plasmids constructed herein contain the necessary signals and controls for efficient expression of the reverse transcriptase gene in bacteria and other microorganisms. The isolated reverse transcriptase gene product is a functional, metabolically stable and biologically active protein.
Claims5
A recombinant plasmid comprising a first DNA sequence derived from an Avian Sarcoma Virus genome and coding for a protein having reverse transcriptase activity, and a second DNA sequence having expression control elements, said recombinant plasmid being capable of replication and directing expression of said DNA sequence in Escherichia coli.
Dependencies are inferred from the claim text.