Modified polysaccharide supports
OwnerKenneth C. Hou, Tung-Ping D. Liaoinventors
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Abstract
A modified polysaccharide material which comprises: (1) polysaccharide covalently bonded to a synthetic polymer; (2) the synthetic polymer being made from (a) a polymerizable compound which is capable of being covalently coupled directly or indirectly to said polysaccharide, and (b) one or more polymerizable compounds containing (i) an ionizable chemcial group, (ii) a chemical group capable of transformation to an ionizable chemical group, (iii) a chemical group capable of causing the covalent coupling of the compound (b) to an affinity ligand or a biologically active molecule or (iv) a hydrophobic compound.
Claims26
A modified polysaccharide material which comprises:
- (1) a water insoluble polysaccharide covalently bonded to a synthetic polymer
- (2) said synthetic polymer comprising a copolymer made from a free-radical polymerization of
- (a) a polymerizable compound containing an epoxy group capable of direct covalent coupling to a hydroxy group of said polysaccharide and a vinyl group, capable of free-radical polymerization;
- and
- (b) a polymerizable compound having the formula ##STR11## wherein R is an alpha, beta-unsaturated polymerizable radical, R' and R" are the same or different C.sub.1 -C.sub.6 alkyl or alkanoyl groups, and R"' is a direct bond or a C.sub.2 -C.sub.3 alkyl group, wherein R' and R" taken together with the N atom may form a heterocyclic ring.
The process of claim 56 wherein said bifunctional cross-linking agent is selected from ##STR14## where Hal is halogen atom, or ##STR15## and n is 2 to 12.
A process for effecting chromatographic separation of at least two components of a sample comprising contacting said sample with a self-supporting fibrous matrix comprising a modified polysaccharide material, said modified polysaccharide material comprising:
- (1) a water insoluble polysaccharide covalently bonded to a synthetic polymer;
- (2) said synthetic polymer comprising a copolymer made from a free-radical polymerization of
- (a) a polymerizable compound containing an epoxy group capable of direct covalent coupling to a hydroxy group of said polysaccharide and a vinyl group, capable of free-radical polymerization;
- and
- (b) a polymerizable compound having the formula ##STR16## wherein R is an alpha, beta-ethylenically unsaturated polymerizable radical, R' and R" are the same or different C.sub.1 -C.sub.6 alkyl or alkanoyl groups, and R"' is a direct bond or a C.sub.2 -C.sub.3 alkyl group, wherein R' and R", taken together with the N atom may form a heterocyclic ring.
Dependencies are inferred from the claim text.