Vaccines for gram-negative bacteria
AssigneeMiles Laboratories, Inc.
InventorsGrace C. Tsay, Michael S. Collins
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Abstract
An immunizing composition is disclosed and comprises a detoxified polysaccharide from a gram-negative bacterium covalently coupled to a detoxified protein from said gram-negative bacterium by means of a 4-12 carbon moiety. To prepare the above immunizing agent, the lipid A portion of lipopolysaccharide from a gram-negative bacterium is separated to give a detoxified polysaccharide. Reactive aldehyde groups are generated on the detoxified polysaccharide by selective oxidation. The detoxified polysaccharide is then covalently coupled to a detoxified protein from said gram-negative bacterium by means of a 4-12 carbon moiety having functionalities reactive to the aldehyde groups on the detoxified polysaccharide and to the carboxylic groups on the detoxified protein.
Claims16
An immunizing composition effective against gram-negative bacteria, selected from the group consisting of Pseudomonas aeruginosa, Proteus sp, Serratia sp, and Kiebsiella sp, free from toxicity and endotoxin activity comprising a detoxified protein containing reactive carboxylic acid groups, derived from said gram-negative bacteria, covalently coupled by means of a 4-12 carbon moiety containing at least two amino groups to a lipid A-free polysaccharide having reactive aldehyde groups, wherein there is used about 3-20 parts of amine per 1 part of detoxified protein and about 1-5 parts of dry lipid A-free polysaccharide per 0.5-3 parts of the foregoing derivatized protein.
An immunizing composition effective against Pseudomonas aeruginosa free from toxicity and endotoxin activity comprising a detoxified protein, selected from the group consisting of (a) a detoxified protein having reactive carboxylic acid groups, derived from Pseudomonas aeruginosa and (b) bovine serum albumin having reactive carboxylic acid groups, covalently coupled by means of a 4-12 carbon moiety containing at least two amino groups to a lipid A-free polysaccharide having reactive aldehyde groups, wherein there is used about 3-20 parts of amine per 1 part of detoxified protein and about 1-5 parts of lipid A-free polysaccharide per 0.5-3 parts of the foregoing derivatized protein.
Dependencies are inferred from the claim text.