Monoclonal antibodies against alveolar surfactant protein
AssigneeCalifornia Biotechology, Inc.
InventorJohn Lewicki
Life of this patent
Estimating the term…
Abstract
Monoclonal antibodies specific for a protein associated with a lung surfactant complex, decreased levels of which are related to respiratory distress syndrome, are disclosed. The antibodies are useful for prediction and diagnosis of respiratory problems in newborns.
Claims15
A hybridoma specific against human alveolar surfactant protein (ASP) which is prepared by fusing an immortalizing cell line with spleen cells derived from a mammal immunized with purified non-human ASP and screened for anti-ASP activity against non-human ASP.
A method for isolating human ASP which comprises fractionating lung fluid from human diagnosed as having alveolar proteinosis by the process which comprises
- (a) repeated centrifugation at high speed to retrieve an ASP-containing pellet;
- (b) extracting lipids from the pellet;
- and
- (c) extracting impurities from the pellet.
The hybridoma DS-1 (ATCC HB8906).
Mab secreted by the hybridoma DS-1.
The method of detecting the presence of human ASP in a biological sample which method comprises:
- (a) immobilizing an Mab specific against human ASP on a solid phase support;
- (b) treating the sample with the immobilized Mab;
- (c) treating the mixture of (b) with a second antibody preparation specific for human ASP;
- and
- (d) detecting the presence of the second antibody preparation fixed to the solid support.
Dependencies are inferred from the claim text.