Diagnosing chlamydia infections with Re-lipopolysaccharide complexed to carrier or antibody thereto
AssigneeOrion Corporation
InventorsPirjo H. Makela, Maija K. Leinonen, Marjatta H. Nurminen-Kalliokoski, Pekka A. I. Saikku
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Abstract
A preparation for the detection of chlamydial infections using lipopolysaccharide of Re-lipopolysaccharide mutants of gram-negative bacteria. The lipopolysaccharide preparation is used in the production of group-specific antibodies to chlamydiae for diagnostic purposes or for the demonstration of antibodies to chlamydial group antigen in specimens.
Claims26
A preparation for diagnosing chlamydial infections which comprises a Re-lipopolysaccharide complexed to a carrier molecule to enhance immunological response, said Re-lipopolysaccharide being isolated from a Re-lipopolysaccharide mutant of a gram-negative bacterium, wherein said Re-lipopolysaccharide is capable of cross-reacting with chlamydial group-specific antibody as may be present in a tested patient fluid sample and thereby indicating the presence of such chlamydial group-specific antibody.
A method for detecting chlamydial infections, said method comprising the steps of:
- (a) contacting a given amount of a Re-lipopolysaccharide or an anti-Re-lipopolysaccharide antibody, said Re-lipopolysaccharide being isolated from a Re-lipopolysaccharide mutant of gram-negative bacteria, said anti-Re-lipopolysaccharide antibody being produced by immunizing animals with Re-lipopolysaccharide, with a sample to be assayed, and
- (b) assaying the contacted sample to determine whether the sample contains chlamydial group-specific antibody which cross-reacts with the Re-lipopolysaccharide or chlamydial group-specific antigen which cross-reacts with anti-Re-lipopolysaccharide antibody.
A preparation for diagnosing chlamydial infections which comprises a antibody to the complex of a Re-lipopolysaccharide and a carrier molecule to enhance immunological response, said anti-Re-lipopolysaccharide complex antibody being produced by immunizing animals with Re-lipoplysaccharide complexed to a carrier molecule to enhance immunological response, and wherein said anti-Re-lipopolysaccharide complex antibody is capable of cross-reacting with chlamydial group-specific antigen as may be present in a tested patient fluid sample and thereby indicating the presence of such chlamydial group-specific antigen.
Dependencies are inferred from the claim text.