Cell culturing apparatus with improved stirring and filter means

An apparatus for culturing cells in a liquid medium comprising

• a fuild tight culture vessel for containing a quantity of said medium and cells,
• a filter head disposed within said vessel and having a filter media with openings smaller than the cells to be cultured,
• flexible conduit means having one end fixed in fluid communication with said filter head for permitting liquid medium in said vessel to be drawn through said filter head and directed out of said vessel by passage through said flexible conduit means,
• means fixedly supporting a portion of said flexible conduit means to said vessel,
• magnetic means carried by said filter head,
• magnetic drive means located outside said vessel for moving a magnetic field in close relation to said filter head magnetic means for moving said filter head and the end of said flexible conduit means fixed thereto in said vessel for stirring the liquid medium and cells therein without relative movement between said vessel and the fixed portion of said flexible conduit means, and
• means disposed within said flexible conduit means for permitting the introduction of fluids into said vessel simultaneously with drawing of said liquid medium through said filter head and directing it through said flexible conduit means.

An apparatus for culturing cells in a liquid medium comprising

• a fluid tight culture vessel for containing a quantity of said medium and cells,
• a stirring head,
• flexible conduit means suspending said stirring head within said vessel and having one end fixed in fluid communication with said stirring head for permitting liquid medium in said vessel to be drawn through said filter head and directed out of said vessel by passage through said flexible conduit means,
• means fixedly securing a portion of said flexible conduit means to said vessel,
• magnetic means carried by said stirring head, and
• magnetic drive means located outside said vessel for moving a magnetic field in close relation to said stirring head magnetic means for moving said stirring head within said vessel for stirring the liquid medium and cells therein without relative movement between said vessel and said fixed portion of said flexible conduit means.