Radioimmunoassays for the serum thymic factor (FTS)
AssigneeSloan-Kettering Institute for Cancer
InventorsBruce W. Erickson, Kam-Fook Fok, Genevieve S. Incefy, Kazuhiro Ohga
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Abstract
Radioimmunossays for the quantitation of serum thymic factor (FTS), a thymic peptide hormone, are disclosed. Each assay employs an antibody specific for FTS, the monoclonal antibody or the antibody from the antiserum of a host animal; synthetic FTS or FTS analogue as the hormone standard; and a radiolabeled FTS analogue as the tracer. Also disclosed is a method of treating serum or other biological fluid prior to assay of FTS to remove interfering substances.
Claims10
Radioimmunoassay of serum thymic factor (FTS) in a test sample, comprising:
- (a) contacting a first aliquot of said sample with anti-FTS antibody, with a known amount of FTS hormone standard and a known amount of radiolabeled FTS analogue;
- and
- (b) contacting a second aliquot of said sample with anti-FTS antibody and a known amount of radiolabeled FTS analogue;
- and
- (c) measuring the radioactivity of the antigen-antibody complex in each aliquot;
- and
- (d) calculating the amount of FTS in the test sample, wherein the hormone standard and the radiolabeled analogue are of the formula ##STR4## wherein X is .sup.125 I for the radiolabeled analogue or hydrogen for the hormone standard and Y is hydrogen or a compound of the formula ##STR5## wherein n is 1 to 4 and Z.sub.1 and Z.sub.2 are hydrogen and/or halogen;
- A is hydrogen or NH.sub.2 ;
- B is alanine or another amino acid;
- and m is an integer from zero to 2.
FTS analogues having the formula:
- ##STR6## wherein X is .sup.125 I and Y is hydrogen or a compound of the formula:
- ##STR7## wherein n is 1 to 4 and Z.sub.1 and Z.sub.2 are hydrogen and/or halogen. A is hydrogen or NH.sub.2 ;
- B is alanine or other amino acid;
- and m is an integer from zero to 2.
Dependencies are inferred from the claim text.