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US 4,629,691 AGranted
Tricyclic antidepressant conjugates with antigens and enzymes
AssigneeSyntex (U.S.A.) Inc.
InventorsChristine G. Collins, Marcel R. Pirio, Prithipal Singh
Life of this patent
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Abstract
Tricyclic antidepressant functionalized compounds are provided for conjugation through a side chain to antigenic compounds, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies and together with the enzyme conjugates find particular use in immunoassays for the determination or detection of the total amount of tricyclic antidepressants present in a sample.
Claims20
A compound of the formula:
- ##STR9## wherein:
- .beta.-.gamma. is N-CH.sub.2 or C.dbd.CH;
- Y is an oxygen or sulfur atom or imino;
- R is a linking group of from 3 to 10 atoms other than hydrogen comprising carbon, nitrogen, oxygen and sulfur containing an alkylene or alkenylene chain of at least 3 carbon atoms linked to the nitrogen atom of the annular side chain;
- when Z is an enzyme, R is a linking group of 4 to 10 atoms other than hydrogen having a chain of at least 4 atoms;
- m is 0 to 1, being 1 when Z is other than poly(amino acid);
- Z is a hydrogen atom, oxy, an activated ester capable of amide formation with amino groups of a poly(amino acid) in an aqueous medium, or an antigenic poly(amino acid) or enzyme, with the proviso that, if .beta.-.gamma. is C.dbd.CH, m is 1, Y is oxygen, and Z is an antigenic poly(amino) acid, then Z is bonded to the carbonyl group by an amide linkage;
- and
- n is 1 when Z is other than a poly(amino acid) and is otherwise a number on the average between 1 and the molecular weight of Z divided by 500.
6 more elements
A compound of the formula:
- ##STR10## wherein:
- Y is oxygen, sulfur or imino;
- m is 0 or 1;
- R' is an aliphatic linking group of from 3 to 6 carbon atoms;
- Z.sup.2 is a poly(amino acid) other than an enzyme
- n.sup.1 is a number on the average between 1 and the molecular weight of Z.sup.2 divided by 500.
3 more elements
A compound of the formula:
- ##STR11## wherein:
- Y is an oxygen or sulfur atom or imino;
- P is 0 or 1;
- R.sup.2 is an aliphatic linking group of from 3 to 4 carbon atoms;
- R.sup.3 is an aliphatic linking group of from 1 to 3 carbon atoms;
- being 3 carbon atoms when p is 0;
- m is 0 or 1;
- Z.sup.3 is an enzyme;
- and
- n.sup.2 is a number on the average between 1 and the molecular weight of Z.sup.3 divided by 500.
7 more elements
Dependencies are inferred from the claim text.