Back to results
US 4,627,982 AGranted
Partially purified bone-inducing factor
AssigneeCollagen Corporation
InventorsSaeid Seyedin, Thomas Thomas
Life of this patent
Estimating the term…
Abstract
A partially purified proteinaceous bone-inducing factor of 10,000 to 30,000 daltons is described. It is derived from demineralized bovine bone by extraction with a chaotropic agent, gel filtration, cation exchange chromatography using carboxymethyl cellulose at pH 4.8 and gradient elution with NaCl at 10 mM to about 150 mM.
Claims8
A process for preparing a partially purified bone-inducing factor which comprises:
- (a) treating demineralized bone with a chaotropic extractant that solubilizes nonfibrous proteins;
- (b) subjecting the extract from step (a) to gel filtration to recover a fraction containing proteins of molecular weight 10,000-30,000;
- (c) adsorbing the fraction from step (b) onto a carboxymethyl cellulose cation exchanger at approximately pH 4.5-5.5;
- and
- (d) eluting an osteogenically active fraction from the cation exchanger with a sodium chloride gradient of about 10 mM to about 150 mM in the presence of a nonionic chaotropic agent.
2 more elements
Dependencies are inferred from the claim text.