Chromatography column using horizontal flow
AssigneeSepragen Corporation
InventorVinit Saxena
Life of this patent
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Abstract
A method and apparatus for performing chromatographic separations utilizing horizontal flow through the separating medium bed are disclosed. The chromatographic separations may be carried out by using an apparatus involving a cylindrical column construction wherein the horizontal flow is in a radial direction as it passes through the separating medium bed or an apparatus involving a cubical column construction wherein the flow traverses from one end of the separating medium bed to the other end horizontally. The apparatus includes means for distributing the flow, achieving horizontal streamlines and collecting separated components. In one embodiment, the separating medium bed in adjustable in height.
Claims32
A chromatography column utilizing horizontal flow of sample material passing therethrough comprising:
- a housing defining a chamber therein and including at least one removable end section,
- a pair of longitudinally extending porous frits positioned in spaced relation within said chamber of said housing,
- a bed of chromatographic separation material positioned in said chamber of said housing and intermediate said porous frits,
- one of said porous frits being spaced from said housing to define an inlet channel therebetween,
- another of said porous frits being positioned to define an outlet channel,
- distribution means operatively connected to said inlet channel,
- collector means operatively connected to said outlet channel,
- said distribution means and said inlet channel being constructed to direct associated material to be separated in said bed evenly across a longitudinal length of said bed in a substantially horizontal direction.
An improved method for chromatographic separation of fluid components utilizing horizontal flows, comprising the steps of:
- retaining a bed of chromatographic separation material between a pair of spaced longitudinally extending porous frits,
- forming a fluid inlet channel adjacent to and extending along a longitudinal length of a first of the pair of porous frits,
- forming a fluid component collection channel adjacent to and extending along a longitudinal length of a second of the porous frits,
- providing a fluid inlet distribution means for directing fluid evenly into the fluid inlet channel across an adjacent longitudinal surface of the first of the porous frits,
- providing collection means for directing fluid components from the collection channel, and
- directing fluid to be separated into the inlet channel and through the pair of spaced frits and the bed of chromatographic material along a longitudinal length of the frits and the bed in a horizontal flow path into the collection channel.
Dependencies are inferred from the claim text.