Composition for reducing turbidity in samples of biological fluids
AssigneeWayne State University
InventorsJoseph D. Artiss, Bennie Zak
Life of this patent
Estimating the term…
Abstract
A composition which reduces the turbidity of samples of artificial or naturally occurring biological fluids comprising: a buffering agent; a nonionic surfactant; alpha-cyclodextrin; a lipase; and water. The composition provides a catalyst for and agents which hydrolyze triglycerides present in the sample to glycerol and free fatty acids. Also present in the composition is a complexing agent or agents to render the catalyzed fatty acids water-soluble.
Claims21
A composition for reducing turbidity in biological fluid samples consisting essentially of:
- a buffering agent;
- a lipase-activating nonionic surfactant;
- cyclodextrin;
- a lipase;
- and
- water.
A composition for reducing turbidity in samples of biological fluids consisting essentially of:
- a buffering agent selected from the group consisting essentially of sodium carbonate, sodium bicarbonate, calcium carbonate, sodium hydroxide, potassium phosphate, sodium phosphate or mixtures thereof;
- an ethylene oxide adduct of nonylphenol;
- cyclodextrin selected from the group consisting essentially of alpha-cyclodextrin, beta-cyclodextrin, gamma-cyclodextrin, or mixtures thereof;
- a lipase derived from Chromobacterium viscosum;
- and
- water.
A composition for reducing the turbidity of hemoglobin containing samples consisting essentially of:
- potassium ferricyanide;
- an alkali metal cyanide, selected from the group consisting essentially of potassium cyanide and sodium cyanide;
- sodium bicarbonate;
- an ethylene oxide adduct of a nonylphenol;
- alpha-cyclodextrin;
- a lipase derived from Chromobacterium viscosum;
- and
- water.
Dependencies are inferred from the claim text.