US 4,626,509 AGranted

Culture media transfer assembly

AssigneeData Packaging Corporation

InventorGeorge F. Lyman

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Abstract

A system for transferring liquid to a multi-well culture plate which includes a machine having a frame upon which is mounted a platform for supporting the culture plate, a transfer plate above the platform and a piston plate above the transfer plate. The transfer plate includes a plurality of chambers closed at the top by a diaphragm. The open bottoms of the chambers may be immersed in a source of the liquid to be transferred on the platform such as a culture plate or reservoir, and the piston plate may be selectively inserted into the chambers of the transfer plate against the diaphragm so that the liquid may be drawn into the chambers from the source. The liquid thereafter may be discharged from the chambers into a multi-well culture plate placed on the platform. A shuttle is mounted adjacent the platform for selectively positioning a liquid source and a culture plate in alignment with the transfer plate, and a calibrated metering control is attached to the piston plate to enable the system to measure accurately, repetitively and conveniently the liquid transferred.

Claims7

Dependent claims

In a machine for transferring liquid to a multi-well culture plate having a frame, a support on the frame for a multi-chamber transfer plate which receives the liquid from a source and deposits it in the culture plate, a piston plate having an array of pistons for entering the chambers to draw liquid into and discharge liquid from the chambers, said piston plate and transfer plate being movable with respect to the culture plate, and guide means on the frame for establishing the path of travel of the piston plate;

wherein the improvement comprises said guide means for controlling the movement of the piston plate comprising:
a rotatable shaft on the frame,
a cam on the shaft operatively connected to the piston plate causing the piston plate to follow the cam surface as the cam rotates with the shaft,
a handle operatively secured to the shaft for rotating the shaft on the frame to in turn rotate the cam,
first detent means on the frame adjacent the shaft,
second detent means on the shaft for engaging the first detent means to establish a first reference position for the shaft which represents a starting position for the piston plate with respect to the transfer plate wherein the pistons enter the chambers a limited extent,
third detent means movable on the shaft for engaging the first detent means, for establishing a second reference position for the shaft which represents a volume measurement position for the piston plate with respect to the transfer plate wherein the pistons enter the chambers a greater extent than the first position,
and means for varying the postion of the third detent means with respect to the shaft for changing the volume measurement postion for the piston plate.

5 more elements

Dependencies are inferred from the claim text.