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US 4,626,504 AGranted
DNA transfer vector for gram-negative bacteria
AssigneeLubrizol Genetics, Inc.
InventorsAlfred Puhler, Reinhard Simon
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Abstract
Modified E. coli vectors are disclosed that are mobilizable to a wide range of gram-negative bacteria, but are not self-transmissible. The modified E. coli plasmids replicate only in bacterial strains of the E. coli group. Mobilizer strains of E. coli are also provided, as well as methods for employing the modified E. coli vectors for transposon mutagenesis, site-specific gene transfer, and the construction of DNA libraries.
Claims22
A suicide plasmid that is mobilizable, but not self-transmissible, comprising:
- (a) a first DNA segment containing a replicon functional in E. coli;
- and
- (b) a second DNA segment containing a Mob-site and oriT of a plasmid transferrable by conjugation in the presence of a tra function encoded by a gene that acts in trans and capable of transferring the plasmid to an organism in which said replicon does not function;
- said suicide plasmid not containing a gene providing said tra function.
1 more element
Dependencies are inferred from the claim text.