Recognins and their chemoreciprocals
OwnerSamuel Bogochinventor
Life of this patent
Estimating the term…
Abstract
The process for producing polypeptide products which are useful in tests for malignant cells is disclosed. The polypeptide products produced by this process are also disclosed. The process employs the steps of extraction of acidic fractionation and formation of a precipitate in the quantitative precipitin test and the Ouchterlony gel diffusion test. The product is characterized by being soluble in acidic solution, insoluble in basic solution, having a particular spectrophotometric absorption and an amino acid residue composition of high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.
Claims17
A process for the production of polypeptide products, Recognins, from nerve tissue which comprises extracting normal or diseased animal nerve tissue or cells with a neutral buffer by repeated disruption of the tissue or cells to solubilize protein fractions, separating from the resulting extract of solubilized proteins the fraction having a pK range of from about 1 to 4, and isolating therefrom a product having a molecular weight of from about 3,000 to about 25,000, said products, Recognins, characterized by forming a single line precipitate with their specific antibodies in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, having a spectrophotometric absorption peak wave length of 280 mu and a molecular weight of from about 3,000 to about 25,000, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.
A polypeptide product, Recognin, derived from normal or diseased animal nerve tissue or cells, said product being characterized by effecting the production of Anti-Recognin antibody upon injection into mammalian tissue or fluids, said antibody having a specific affinity, for tumor cells;
- and said Recognin product being further characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, having a spectrophotometric absorption peak wave length of 280 mu and a molecular weight of from 3,000 to about 25,000, being capable of complexing with bromoacetylcellulose, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.
A process for the production of polypeptide products which comprises extracting animal cancer tumor nerve tissue or cells with a neutral buffer by repeated disruption of the tissue or cells to solubilize protein fractions, separating from the resulting extract of solubilized proteins the fraction having a pK range of from about 1 to 4, and isolating therefrom a product having a molecular weight of from about 3,000 to about 25,000, said products, cancer Recognins, characterized by forming a single line precipitate with their specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and in soluble at an alkaline pH, having a spectrophotometric absorption peak wave length of 280 mu and a molecular weight of from about 3,000 to about 25,000, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.
A process for the production of polypeptide products which comprises extracting animal cancer tumor nerve tissue or cells with a neutral buffer by repeated mechanical disruption of the tissue or cells to solubilize protein fractions, concentrating the extracts, separating from the resulting concentrate of solubilized proteins a precursor fraction having a pK range of from about 1 to 4, and isolating therefrom a purified product having a molecular weight of from about 3,000 to about 25,000, said polypeptide products, cancer Recognins, characterized by forming a single line precipitate with their specific antibody in quantitative precipitin tests and Ouchterlony gel diffusin tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, having a spectrophotometric absorption peak wave length of 280 mu and a molecular weight of from about 3,000 to about 25,000, and having an amino acid residue composition characterized by high ratios of glutamic and aspartic acids, high ratios of glutamic and aspartic acids to histidine, and by the absence of cysteic acid, hydroxy-proline, norleucine, ammonia, isodesmosine, desmosine, hydroxylysine, lysinonorleucine and gamma-aminobutyric acid.
Dependencies are inferred from the claim text.