Isolation of pancreatin
AssigneeBASF SE
InventorHans Schultze
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Abstract
Highly active pancreatin is obtained by autolysis of an aqueous isopropanol-containing tissue slurry, preferably buffered with sodium bicarbonate, until a test precipitation with aqueous isopropyl alcohol proves positive, and precipitating the batch with a higher concentration of isopropyl alcohol, resulting in a fiber suspension which can be sieved so that the solution obtained permits direct isolation of pancreatin by further increasing the concentration of isopropyl alcohol. A high bulk density of the finished dry preparation is achieved by stirring the precipitated pancreatin with isopropyl alcohol or acetone so as to bring the pancreatin to a concentration of 70-85% of isopropanol or 80-95% of acetone, isolating the pancreatin by suction filtration or centrifuging and drying it by treatment with dry air or nitrogen.
Claims5
A process for isolating pancreatin by autolysis of an aqueous pancreas tissue suspension, wherein the autolysis is carried out at below 30.degree. C. with addition of up to 20% by weight of isopropyl alcohol, based on the suspension, at a pH of from 6.5 to 8.5, testing a sample of the autolyzed suspension to determine the sedimentation rate and stopping the autolysis as soon as a sample in 55-65% strength aqueous isopropyl alcohol shows a sedimentation rate, measured under gravity, of about 3-10 mm/1-3 minutes.
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