Hepatitis A virus purified and triply cloned
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsRichard J. Daemer, Stephen M. Feinstone, Ian D. Gust, Robert H. Purcell
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Abstract
Human hepatitis A virus (HAV) can be purified by a process in which master seed lots of HM-175 strain of hepatitis A virus are prepared from triply cloned virus by terminal dilution at passage level about 20 or preferably at least 20-30. The clones tested induced minimal or no hepatitis although significant antibody response was provoked in inoculated primates. A method of preparing the triply cloned inoculum is also described.
Claims7
A method of producing a protective antibody response in higher primates by injecting said primate with a hepatitis A live attenuated virus, strain HM-175 injection which is of uniform virus composition.
An improved vaccine for mammals comprising a triple cloned hepatitis A virus, strain HM-175 that is useful after attenuation as a vaccine.
Dependencies are inferred from the claim text.