Alpha-acetolactate decarboxylase enzyme and preparation thereof
AssigneeNovo Industri A/S
InventorsFrank Olsen, Knud Aunstrup
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Abstract
A novel alpha-acetolactate decarboxylase enzyme product with improved stability is provided. The novel enzyme is produced in high levels by cultivation of Bacillus strains selected from the group consisting of Bacillus brevis and Bacillus licheniformis ATCC 11031, ATCC 12759, ATCC 12713, ATCC 11946, ATCC 27326, NRRL B-3751, NCTC 2120, NCTC 8721, NCIB 6816, NCIB 8537 and NCIB 11868.
Claims5
An alpha-acetolactate decarboxylase enzyme obtained by cultivation in a suitable nutrient medium of a Bacillus brevis strain or a Bacillus licheniformis strain productive of an alpha-acetolactate decarboxylase characterized by a half-life of at least about 11 days at 10.degree. C. in beer.
A process for the preparation of an alpha-acetolactate decarboxylase enzyme, which process comprises cultivation in a suitable nutrient medium containing carbon and nitrogen sources and inorganic salts of an alpha-acetolactate decarboxylase producing Bacillus strain selected from the group consisting of Bacillus brevis ATCC 11031, Bacillus licheniformis ATCC 12759, ATCC 12713, ATCC 11946, ATCC 27326, NRRL B-3751, NCTC 2120, NCTC 8721, NCIB 6816, NCIB 8537, NCIB 11868 and mutants and variations thereof productive of the alpha-acetolactate decarboxylase enzyme, and thereafter recovery of the alpha-acetolactate decarboxylase enzyme from the cells in the medium.
Dependencies are inferred from the claim text.