US 4,617,009 A · Method and apparatus for centrifugal batch separation of blood

Abstract

Centrifugal batch separation of blood in a closed collapsible container is carried out by placing the container in a rigid casing in which the container is divided into two or more sections in that the casing constricts the container at one or more predetermined positions between the ends of the container. The casing with the container therein is then centrifuged, a gap-like passage between the container sections permitting rearrangement of the contents of the container so that each section will accommodate a blood fraction. After the centrifugation the fractions are isolated from each other. The volume of the cavity within the casing accommodating the radially outermost container section is matched with the hematocrit and the total blood volume by means of filler bodies.

Claims29

Dependent claims

A method for centrifugal batch separation of blood into fractions of different densities, comprising;

(a) confining a quantity of blood in an elongated closed container having walls of flexible sheet material;
(b) placing the container with the blood therein in a rigid casing;
(c) by means of constriction means within the casing causing the container to be divided into a plurality of sections by forming a container constriction in the opposite container walls extending across the width of the container, one of said sections being an end section for receiving the fraction of the highest density and having a volume determined by the shape and dimensions of the casing;
said container constriction forming a flow passage between said sections;
(d) placing the casing with the container in an eccentric position in a centrifuge and centrifuging it with the end of the container remote from said end section in a radially inner position and said end section in a radially outer position to cause stratification of the blood into said fractions with the interface of adjoining fractions oriented transversely of the longitudinal direction of the container;
and
(e) isolating the fractions from each other within the container by pinching the container along said container constriction to close said flow passage without substantially changing the shape of the container.

4 more elements

A device for use in centrifugal batch separation of blood into fractions of different densities, the blood being contained in an elongated closed blood container having walls of flexible sheet material and filled with a predetermined discrete volume of the blood, comprising:

a rigid, openable casing adapted to be removably mounted in an eccentric position on a centrifuge rotor, said casing defining an elongated container-receiving chamber adapted to confine therein, and to support the container substantially unyieldingly, the container-receiving chamber comprising two end compartments receptive of opposed end sections of the container and separated by a constriction extending transversely across the width of the container-receiving chamber adjacent to the central portion thereof, said constriction being adapted to define a narrow passage of communication within the container between said end compartments, a constricting means comprising at least one pinching device extending across the container-receiving chamber and adapted to engage and pinch the container at the constriction, heat sealing means disposed adjacent to a said pinching device, and means on said casing for connecting said heat sealing means to a source of electrical energy.

Dependencies are inferred from the claim text.

Method and apparatus for centrifugal batch separation of blood

Life of this patent

Method and apparatus for centrifugal batch separation of blood

Life of this patent

Abstract

Claims29