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US 4,614,712 AGranted
Immunoassays with luciferase labeled ligands or receptors
AssigneesThe Upjohn Company, The Texas A&M University System
InventorsThomas O. Baldwin, Thomas F. Holzman, Paul S. Satoh, Frederick S. Yein
Life of this patent
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Abstract
Immunoassays which utilize an enzyme linked ligand or receptor wherein the enzyme is bacterial luciferase; mercantile kit useful in performing said immunoassay; and compounds utilized in performing said assay.
Claims26
A luciferase labeled ligand or a luciferase labeled receptor of the formula
- wherein E represents luciferase absent the reactive sulfhydryl group;
- n is zero to 15;
- R.sub.2 and R.sub.3 are the same and are hydrogen, methyl or ethyl;
- R.sub.4 and R.sub.5 are the same and are hydrogen, methyl or ethyl;
- or R.sub.2, R.sub.3, R.sub.4 and R.sub.5 taken together with the carbon atoms to which they are attached represent a cycloalkyl group having from 4 to 6 carbon atoms, or represent 1,4-phenylene;
- m is an integer equivalent to the number of reactive functions present on the ligand or receptor capable of being labeled;
- X represents a functional linkage selected from the group consisting of ##STR9## --NR-- wherein R is hydrogen or any group which may be present on the ligand or receptor functional secondary amine;
- ##STR10## and Y represents the ligand or receptor to be labeled or assayed.
5 more elements
Dependencies are inferred from the claim text.