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AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsJeffrey Schlom, David Colcher, Marianna Nuti, Patricia H. Hand, Faye Austin
Estimating the term…
Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.
Antibodies from hybridoma cultures produced by the steps of
Antibodies selected from one member of the group consisting of B6.2, B25.2, B72.3, F25.2, B38.1, and B50.4.
Antibodies selected from one member of the group consisting of B6.2, B25.2, B38.1, F25.2, B72.3 and B50.4, which bind human mammary carcinoma lines designated BT-20, MCF-7 and ZR-75-1.
A mouse myeloma cell line selected from the group consisting of ATCC #HB8106, #HB8107, #HB8108, #HB8109, #HB8110, and #HB8111.
A method of detecting a small number of mammary cancer cells in micro-lesions in thin tissue sections or body fluids by applying monoclonal antibodies selected from the group consisting of monoclonal antibodies designated B6.2, B25.2, B72.3, F25.2, B38.1, and B50.4 to the tissue, adding quantity of goat (anti-mouse Ig) antibody conjugated with peroxidase and fixing with diaminobenzidene and peroxide and staining with hemotoxylin, and after fixing, examining for reddish brown colored cells indicative of mammary cancer cells.
In an assay to detect mammary carcinoma cells involving contacting a sample with an antibody under conditions which allow the formation of an antibody antigen complex and measurement of the complex formation, the improvement comprises using monoclonal antibodies selected from the group consisting of B6.2, B25.2, B72.3, F25.2, B38.1, and B50.4.
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