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AssigneesInterthyr Research Foundation, Inc., University of Maryland
InventorsLeonard D. Kohn, William A. Valente, Evelyn F. Grollman-Wolff, Salvatore M. Aloj, Paolo Vitti
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A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is cloned from FRTL cells obtained from primary cultures of Fischer rat thyroid glands. The FRTL-5 cells are cultured in a medium containing approximately 5 percent calf serum supplemented with a mixture of hormones, at least one of which is thyrotropin. The FRTL-5 cells are employed in a series of assays which measure thyroid stimulatory or inhibitory factors. The FRTL-5 system of assay specifically measures thymidine incorporation, cAMP elevation and iodide uptake and permits the evaluation of patient sera, particularly those afflicted with Graves' disease and other autoimmune thyroid diseases, thereby providing a means for determining appropriate methods of treatment.
An assay method for measuring thyroid stimulatory or inhibitory factors by determining the ability of said thyroid stimulatory or inhibiting factors to cause or prevent, respectively, incorporation of thymidine in a continuous strain of FRTL-5 ATCC CRL 8305 rat thyroid cells capable of surviving thyrotropin withdrawal for a period of up to about 10 days comprising the sequential steps of:
An assay method for measuring thyroid stimulatory or inhibitory factors by determining the ability of said thyroid stimulatory or inhibitory factors to cause or prevent, respectively, cAMP elevation in a continuous strain of functional FRTL-5 ATCC CRL 8305 rat thyroid cells capable of surviving thyrotropin withdrawal for a period of up to about 10 days comprising the sequential steps of:
An assay method for measuring thyroid stimulatory or inhibitory factors by determining the ability of said thyroid stimulatory or inhibitory factors to cause or prevent, respectively, cAMP mediated iodine uptake by a continuous strain of functional FRTL-5 ATCC CRL 8305 rat thyroid cells capable of surviving thyrotropin withdrawal for a period of up to about 10 days comprising the sequential steps of:
An assay method for measuring thyroid stimulatory or inhibitory factors by determining the ability of said thyroid stimulatory or inhibitory factors to cause changes in growth, function or concentration of cAMP present in a continuous strain of FRTL-5 ATCC CRL 8305 rat thyroid cells capable of surviving thyrotropin withdrawal for a period of up to about 10 days comprising the sequential steps of:
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