LHRH analogues useful in stimulating anti-LHRH antibodies and vaccines containing such analogues
AssigneePitman-Moore, Inc.
InventorAbdus S. Mia
Life of this patent
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Abstract
A conjugate between a nona- or decapeptide of the formula (i) or (ii): Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2, (i) or Cys-Lys-Trp-Ser-Try-Gly-Leu-Arg-Pro-Gly-NH.sub.2, (ii) or mixtures of peptides (i) and (ii). and a protein is provided which, when used per se or when mixed with a suitable adjuvant, yields a vaccine which acts as an immunogen for LHRH and induces a mammal to produce antibodies which react with LHRH. Immunization against the body's LRHR results in lowering of male and female sex hormones including luteinizing hormone so as to prevent conception. Other uses for materials which lessen the effect of LHRH in the body are known in the art.
Claims29
An immunogen vaccine which induces in a manmal production of antibodies against LHRH, which vaccine comprises a water in oil emulsion, the water phase comprising conjugate between a protein and a biologically effective amount of a peptide selected from the group consisting of:
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or mixtures of
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- and
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2.
A method of inducing in a mammal the production of antibodies to LHRH which comprises administering to the mammal an immunogen vaccine which induces in the mammal production of antibodies against LHRH, which vaccine comprises a water in oil emulsion, the water phase comprising a conjugate between a protein and a biologically effective amount of a peptide selected from the group consisting of:
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or mixtures of
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- and
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2.
A method of preventing ovulation in a mammal which comprises administering to the mammal an immunogen vaccine which induces in the mammal Production of antibodies against LHRH, which vaccine comprises a water in oil emulsion, the water phase comprising a conjugate between a protein and a biologically effective amount of a peptide selected from the group consisting of:
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or mixtures of
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- and
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2.
A method of treating a mammal for cryptorchidism which comprises administering to the mammal an immunogen vaccine which induces in the mammal production of antibodies against LHRH, which vaccine comprises a water in oil emulsion, the water phase comprising a conjugate between a protein and a biologically effective amount of a peptide selected from the group consisting of:
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or mixtures of
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- and
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2.
A peptide selected from the group consisting of:
- Lys-Trp-Ser-Try-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- and ##STR2##
A conjugate between a protein having a molecular weight of at least about 40,000 and a peptide selected from the group consisting of:
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- or mixtures of
- Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2,
- and
- Cys-Lys-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH.sub.2.
Dependencies are inferred from the claim text.