Lipoprotein lipase suppression by endotoxin-induced mediator (shock assay)

An antibody to a mediator present in the serum of a mammal which has been subjected to invasive stimuli, the mediator to which said antibody is raised comprises a proteinaceous mediator originating from invasive stimuli treated macrophage cells having the following characteristics;

• capable of suppressing the activity of the anabolic enzymes lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the differentiation of erythroid-committed cells.

A method for detecting the presence of invasive stimuli in mammals comprising measuring the activity of a proteinaceous mediator originating from invasive stimuli treated macrophage cells having the following characteristics;

• capable of supporessing the activity of lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

The method of claim:

• 26 wherein at least one member of said second generation of antibodies is included in said labeled material.

A method of testing the ability of a drug to inhibit the activity of a mediator capable of reducing the lipoprotein lipase activity in the nammalian body which comprises culturing 3T3-L1 cells in a growth media containing the mediator, adding the drug under test and thereafter testing lipoprotein lipase activity of the media, said mediator comprising a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

• capable of suppressing the activity of the anabolic enzymes lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

An assay system for screening drugs and other agents for ability to inhibit production and/or activity of a mediator comprising an observable cellular test colony innoculated with said mediator said mediator being a proteinaceous mediator originating from invasive stimuli treated macrophase cells having the following characteristics;

• capable of suppressing the activity of lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

A kit useful for detecting the presence in a mammal of a mediator which is capable of reducing lipoprotein lipase activity which comprises at least a mediator substance labeled with a detectable label, wherein said mediator comprises a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

• capable of suppressing the activity of the anabolic enzymes lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.

A kit useful for detecting the presence in a mammal of a mediator which is capable of reducing lipoprotein lipase activity which comprises at least a mediator antibody labeled with a detectable label, said mediator comprising a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

• capable of suppressing the activity of lipoprotein lipase, acetyl coenzyme A carboxylase and fatty acid snythetase, and capable of inhibiting the growth and differentiation of erythroid-committed cells.

A kit useful for detecting the presence in a mammal of a mediator which is capable of reducing lipoprotein lipase activity which comprises at least a mediator antibody and a second antibody which is labeled with a detectable label and is reactive with said mediator antibody, said mediator comprising a protein material originating from invasive stimuli treated macrophage cells having the following characteristics;

• capable of suppressing the activity of lipoprotein lipase, acetyl Coenzyme A carboxylase and fatty acid synthetase, and inhibiting the growth and differentiation of erythroid-committed cells.