Mammalian metallothionein promoter system
AssigneeThe Regents of the University of California
InventorMichael Karin
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Abstract
A cloning vector comprises a replication system from a bovine papilloma virus, a regulatory system including the promoter and terminator from a human metallothionein II gene, and a prokaryotic replication system. The vector is useful for transforming mammalian cells where it is maintained as an automonously replicating episome. Transcription may be regulated by the presence of heavy metals and/or glucocorticoid hormones in the growth media.
Claims16
A DNA sequence of less than 500 base pairs, said DNA sequence comprising the human MT-II transcriptional regulatory system further comprising the transcription initiation sequence.
A DNA construct capable of regulated expression of an inserted gene in a mammalian host, said construct comprising an extrachromosomal replication system recognized by a mammalian host, which replication system has been joined to a human MT-II gene inducible regulatory system in vitro, said construct having DNA sequence(s) defining at least one restriction site within the transcriptional control of the regulatory region for insertion of the gene.
A DNA construct useful for expression of a structural gene to produce a polypeptide in a mammalian host, said vector comprising (a) a replicon from bovine papilloma virus and (b) a regulatory system from a human MT-II gene comprising a promoter and a terminator and having at least one restriction site downstream from the promoter in the direction of transcription.
Dependencies are inferred from the claim text.