Immobilized cells for preparing phenylalanine
AssigneePurification Engineering, Inc.
InventorsLouis L. Wood, Gary J. Calton
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Abstract
A process is disclosed for preparing phenylalanine which comprises contacting phenylpyruvic acid or phenylpyruvate with immobilized whole cells having transaminase activity in the presence of an amine donor. The cells are preferably immobilized with a polyazetidine polymer. Ruptured or permeabilized cells, with the enzyme in the free or immobilized state, may also be used. The preparation of phenylalanine from cinnamic acid using immobilized cells having phenylalanine ammonia lyase activity is also disclosed.
Claims17
A process for preparing phenylalanine which comprises preparing an aqueous reaction mixture consisting essentially of phenylpyruvic acid or phenylpyruvate, pyridoxal -5-phosphate as a cofactor and an amine donor selected from the group consisting of L-glutamic acid and L-aspartic acid, and contacting said reaction mixture with cells immobilized with a polyazetidine polymer, the cells having transaminase activity to obtain said phenylalanine in high yield.
The process of claim 4 wherein the excess of amine donor is at least 50% and the pH is 5-10.
A process for preparing phenylalanine which comprises preparing an aqueous reaction mixture consisting essentially of phenylpyruvic acid or phenylpyruvate, pyridoxal -5-phosphate as a cofactor and an amine donor selected from the group consisting of L-glutamic acid and L-aspartic acid, and contacting said reaction mixture with cells having transaminase activity to obtain said phenylalanine in high yield, said cells being immobilized with a polyazetidine polymer and having been ruptured or permeabilized in order to increase contact between the active enzyme of said cells and the phenylpyruvic acid or phenylpyruvate.
A composition comprising immobilized cells having transmaminase activity, an amine donor, and phenylpyruvic acid or phenylpyruvate, said cells being immobilized with a polyazetidine polymer.
A composition suitable for use in converting phenylpyruvic acid or phenylpyruvate to phenylalanine comprising immobilized cells having transaminase activity, said cells being immobilized with a polyazetidine polymer.
A process for preparing phenylalanine which comprises contacting cinnamic acid with immobilized cells having phenylalanine ammonia lyase actibity, said cells being immobilized with a polyazetidine polymer.
Dependencies are inferred from the claim text.