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US 4,593,002 AGranted
Viruses with recombinant surface proteins
AssigneeThe Salk Institute Biotechnology/Industrial Associates, Inc.
InventorRenato Dulbecco
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AssigneeThe Salk Institute Biotechnology/Industrial Associates, Inc.
InventorRenato Dulbecco
Estimating the term…
Foreign protein segments having specific medically or commercially useful biological functions are incorporated in surface proteins of viruses. The viruses with the incorporated protein segments are convenient agents for introducing the protein segments into animals, such as humans, and are thus useful as vaccines. Small segments of an original protein exhibiting desired functions are identified, and a DNA fragment having a nucleotide base sequence encoding that segment of the protein is isolated from an organism or synthesized chemically. The isolated DNA fragment is inserted into the DNA genome of a virus in a manner such that the inserted DNA fragment expresses itself as the foreign segment of a surface viral protein and in such a way that neither the function of the protein segment nor the function of any viral protein critical for viral replication is impaired.
A method of modifying a virus to give the virus a new biological function comprising inserting a foreign nucleotide base sequence into the viral genome at a location whereat said foreign nucleotide base sequence expresses itself as an exposed segment of a surface viral protein, which surface viral protein forms a part of the coat of the modified virus.
A method of modifying a virus to produce a modified virus for introduction into a live animal comprising selecting for modification a virus which is known to be non-pathogenic in the animal into which said modified virus is to be introduced, and inserting a foreign nucleotide base sequence into the viral genome of said selected virus at a location whereat said foreign nucleotide base sequence is expressed as an exposed segment of a surface viral protein, which expressed surface viral protein forms a part of the coat of the modified virus.
A method of producing an agent which induces an immunological response in an animal comprising
A method of producing a modified virus which carries an exposed segment of a foreign protein, which segment is known to be immunologically active within an animal into which the modified virus is to be introduced, the method comprising
Dependencies are inferred from the claim text.