Human recombinant cysteine depleted interferon-.beta. muteins
AssigneeCetus Corporation
InventorsDavid F. Mark, Leo S. Lin, Shi-Da Yu Lu
Life of this patent
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Abstract
Muteins of biologically active proteins such as IFN-.beta. and IL-2 in which cysteine residues that are not essential to biological activity have been deleted or replaced with other amino acids to eliminate sites for intermolecular crosslinking or incorrect intramolecular disulfide bridge formation. These muteins are made via bacterial expression of mutant genes that encode the muteins that have been synthesized from the genes for the parent proteins by oligonucleotide-directed mutagenesis.
Claims10
Recombinant, synthetic human interferon-.beta. mutein, wherein the cysteine at position 17, numbered in accordance with native interferon-.beta., is deleted or replaced by a neutral amino acid, and wherein said mutein exhibits biological activity of native, human interferon-.beta..
Biologically active IFN-.beta..sub.ser17.
IFN-.beta..sub.ser17 as represented by the amino acid sequence represented in FIG. 10.
Dependencies are inferred from the claim text.