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US 4,575,452 AGranted
Kit for silver staining proteins and nucleic acids
AssigneeE. I. Du Pont de Nemours and Company
InventorsDuk H. Lee, Thomas J. O'Connell, III
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Abstract
A method and kit for the optical detection of proteins and nucleic acids in a matrix, such as polyacrylamide electrophoresis gels. The method comprises fixing the proteins and nucleic acids in the matrix using aromatic sulfonic acids having tertiary amines capable of forming coordination complexes with silver ion.
Claims3
A kit for the optical detection of proteins and nucleic acids in a matrix, comprising:
- (a) a fixer comprising a compound selected from the group consisting of ##STR16## wherein R is H, CH.sub.3, C.sub.2 H.sub.5 or CH.sub.2 N.sup.+ (CH.sub.3).sub.3, ##STR17## (b) a sensitizer selected from the group consisting of sodium sulfide, thiourea, dithiothreitol and sodium thiosulfate;
- (c) a source of silver ions;
- (d) a developer capable of reducing silver ions to metallic silver;
- and
- (e) a stopper capable of stopping reduction of silver ions to metallic silver.
2 more elements
Dependencies are inferred from the claim text.