Monoclonal antibodies specific for the unbound .beta. subunit of human chorionic gonadotropin
AssigneeThe Reagents of the University of Michigan
InventorsMohammad B. Khazaeli, William H. Beierwaltes, Barry G. England
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Abstract
Hybrid-myeloma cells which produce monoclonal antibody specific to the .beta. subunit of human chorionic gonadotropin and methods of use of the monoclonal antibody.
Claims11
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound .beta. subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
A murine hybridoma produced by the fusion of a murine lymphocyte and a myeloma murine cell line that produces and secretes monoclonal antibodies of the IgG class that substantially specifically bind to the unbound .beta. subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell that produces and secretes monoclonal antibodies of the IgC class that specifically bind to the unbound .beta. subunit of human chorionic gonadotropin in the presence of the .beta. subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone as intact glycoprotein molecules.
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound .beta. subunit of human chorionic gonadotropin having substantially no inhibition in said binding in the presence of unbound .beta. subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone.
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound .beta. subunit of human chorionic gonadotropin having substantially no cross reactivity with intact human luteinizing hormone, human thyroid stimulating hormone and human follicle stimulating hormone and less than 0.25% cross reactivity with intact human chorionic gonadotropin and having substantially no inhibition in said binding in the presence of unbound .beta. subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human follicle stimulating hormone at up to 250 ng/tube.
The method of detecting a predominantly .beta.-human chorionic gonadotropin (.beta.-hCG) subunit secreting tumor in vitro, comprising:
- reacting unbound .beta.-human chorionic gonadotropin (.beta.-hCG) in sera with murine monoclonal antibody of the IgG class specific to said .beta.-hCG in the presence of intact human chorionic gonadotropin and determining the amount of .beta.-hCg-monoclonal antibody.
The method of detecting unbound .beta.-human chorionic gonadotropin (.beta.-hCG) by binding said unbound .beta.-hCG to murine monoclonal antibody of the IgG class specific to said .beta.-hCG in the presence of intact human chorionic gonadotropin by means of affinity chromatography and then determining the amount of .beta.-hCG-monoclonal antibody.
The method of radioimmunodetecting a tumor secreting unbound .beta.-human chorionic gonadotropin subunit (.beta.-hCG) comprising contacting said tumor with radiolabeled murine monoclonal antibody of the IgG class specific to said unbound (.beta.-hCG) in the presence of intact human chorionic gonadotropin and detecting the radioactivity emitted.
The method of therapeutically treating a tumor rich in unbound .beta.-human chorionic gonadotropin subunits (.beta.-hCG) comprising contacting said tumor with a therapeutic amount of radiolabeled murine monoclonal antibody of the IgG class specific to said unbound .beta.-hCG in the presence of intact human chorionic gonadotropin.
The hybridoma cell line having the essential characteristics of hybridoma cell line KEB-1E5.1, deposited as ATCC HB8095, said characteristics consisting essentially of the production of murine monoclonal antibodies which specifically bind to the unbound .beta.-human chorionic gonadotropin subunit in the presence of intact human chorionic gonadotropin and in the presence of the .beta. subunit of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone.
A murine antibody of the IgG class that specifically binds to the unbound .beta. subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
Dependencies are inferred from the claim text.