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US 4,563,349 AGranted
Superoxide dismutase, its immobilized form, and their production and use
AssigneeTakeda Chemical Industries, Ltd.
InventorsKouichi Miyata, Kazutaka Maejima, Katsumi Tomoda
Life of this patent
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Abstract
A novel superoxide dismutase is produced by cultivating a microorganism belonging to the genus Serratia. The superoxide dismutase is useful as an antiinflammatory agent. An immobilized superoxide dismutase has properties of decreased antigenicity and increased antiinflammatory activity.
Claims9
Superoxide dismutase having the following properties:
- (a) Activity:
- to dismutate a superoxide ion into a hydrogen peroxide molecule and an oxygen molecule;
- (b) Substrate specificity:
- to act on superoxide ion;
- (c) Optimum pH range:
- about 7 to 8;
- (e) Stable pH range:
- about 6 to 11;
- (e) Optimum functional temperature range:
- about 20.degree. to 45.degree. C.;
- (f) Thermostability:
- stable at 37.degree. C. for 60 minutes;
- Inactivated about 50% at 50.degree.-60.degree. C. in 60 minutes;
- Inactivated about 100% at 80.degree. C. in 5 minutes;
- (g) Molecular weight:
- about 4.8.times.10.sup.4 (by gel filtration method);
- (h) Elemental analysis:
- C. 48.90.+-.2.0%, H. 7.05.+-.2.0%, N. 14.80.+-.2.0%,
- (i) Absorptions in the visible region of the spectrum;
- 470-475 nm (max.), 375-380 nm (min.), about 610 nm (shoulder);
- (j) Molar extinction coefficient:
- about 1.08.times.10.sup.5 M.sup.-1.cm.sup.-1 at 280 nm;
- (k) Amino acid analysis:
- ______________________________________ Amino acid Number of residues ______________________________________ Glycine 23.5 Leucine 28.3 ______________________________________
- wherein the values denote the number of amino acid residues per molecule of the enzyme.
22 more elements
An antiinflammatory agent which contains an effective amount of superoxide dismutase having the following properties:
- (a) Activity:
- to dismutate a superoxide ion into a hydrogen peroxide molecule and an oxygen molecule;
- (b) Substrate specificity:
- to act on superoxide ion;
- (c) Optimum pH range:
- about 7 to 8;
- (d) Stable pH range:
- about 6 to 11;
- (e) Optimum functional temperature range:
- about 20.degree. to 45.degree. C.;
- (f) Thermostability:
- Stable at 37.degree. C. for 60 mintues;
- Inactivated about 50% at 50.degree.-60.degree. C. in 60 minutes;
- Inactivated about 100% at 80.degree. C. in 5 minutes;
- (g) Molecular weight:
- about 4.8.times.10.sup.4 (by gel filtration method);
- (h) Elemental analysis:
- C. 48.90.+-.2.0%, H. 7.05.+-.2.0%, N. 14.8.+-.2.0%,
- (i) Absorptions in the visible region of the spectrum:
- 470-475 nm (max.), 375-380 nm (min.), about 610 nm (shoulder);
- (j) Molar extinction coefficient:
- approx. 1.08.times.10.sup.5 M.sup.-1. cm.sup.-1 at 280 nm;
- (k) Amino acid analysis:
- ______________________________________ Amino acid Number of residues ______________________________________ Glycine 23.5 Leucine 28.3 ______________________________________
- wherein the values denote the number of amino acid residues per molecule of the enzyme,
- as an active ingredient in association with a pharmaceutically acceptable carrier or excipient therefor.
23 more elements
An immoblized superoxide dismutase which is produced by reacting superoxide dismutase having the following properties:
- (a) Activity:
- to dismutate a superoxide ion into a hydrogen perioxide molecule and an oxygen molecule;
- (b) Substrate specificity:
- to act on superioxide ion;
- (c) Optimum pH range:
- about 7 to 8;
- (d) Stable pH range:
- about 6 to 11;
- (e) Optimum functional temperature range:
- about 20.degree. to 45.degree. C.;
- (f) Thermostability:
- stable at 37.degree. C. for 60 minutes;
- Inactivated about 50% at 50.degree.-60.degree. C. in 60 minutes;
- Inactivated about 100.degree. at 80.degree. C. in 5 minutes;
- (g) Molecular weight:
- about 4.8.times.10.sup.4 (by gel filtration method);
- (h) Elemental analysis:
- C. 48.90.+-.2.08%, H. 7.05.+-.2.08%, N. 14.80.+-.2.08%,
- (i) Absorptions in the visible region of the spectrum:
- 470-475 nm (max.), 375-390 nm (min.), about 610 nm (shoulder);
- (j) Molar extinction coefficient:
- about 1.08.times.10.sup.5 M.sup.-1. cm.sup.-1 at 280 nm;
- (k) Amino acid analysis:
- ______________________________________ Amino acid Number of residues ______________________________________ Glycine 23.5 Leucine 28.3 ______________________________________
- the values denote the number of amino acid residues per molecule of the enzyme,
- with a water-soluble carrier.
23 more elements
An antiinflammatory agent which contains an effective amount of an immbolized superoxide dismutase which is produced by reacting superoxide dismutase having the following properties:
- (a) Activity:
- to dismutate a superoxide ion into a hydrogen peroxide molecule and an oxygen molecule;
- (b) Substrate specificity:
- to act on superoxide ion;
- (c) Optimum pH range:
- about 7 to 8;
- (d) Stable pH range:
- about 6 to 11;
- (e) Optimum functional temperature range:
- about 20.degree. to 45.degree. C.;
- (f) Thermostability:
- Stable at 37.degree. C. for 60 minutes;
- Inactivated about 50% at 50.degree.-60.degree. C. in 60 minutes;
- Inactivated about 100% at 80.degree. C. in 5 minutes;
- (g) Molecular weight about 4.8.times.10.sup.4 (by gel filtration method);
- (h) Elemental analysis:
- C. 48.90.+-.2.0%, H. 7.05.+-.2.0%, N. 14.80.+-.2.0%,
- (i) Absorptions in the visible region of the spectrum:
- 470-475 nm (max.), 375-380 nm (min.), about 610 nm (shoulder);
- (j) Molar extinction coefficient:
- approx. 1.08.times.10.sup.5 M.sup.-1. cm.sup.-1 at 280 nm;
- (k) Amino acid analysis:
- ______________________________________ Amino acid Number of residues ______________________________________ Glycine 23.5 Leucine 28.3 ______________________________________
- wherein the values denote the number of amino acid residues per molecule of the enzyme,
- with a water-soluble carrier, as an active ingredient in association with a pharmaceutically acceptable carrier or excipient therefor.
22 more elements
A method of producing superoxide dismutase characterized by cultivating Serratia marcescens ATCC 21074 in a culture medium to thereby cause the microorganism to elaborate and accumulate superoxide dismutase in said medium and harvesting said superoxide dismutase from the resulting culture broth.
Dependencies are inferred from the claim text.