Serum-free cell culture medium and process for making same
AssigneeImmunex Corporation
InventorPaul E. Baker
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Abstract
A defined serum-free medium that is capable of growing a wide range of suspension and monolayer cells includes a serum substitute composed of fetuin, transferrin, phosphatidylcholine, e.g., 1-oleoyl-2-palmitoyl-phosphatidylcholine, linoleic acid and cholesterol. The medium also includes various inorganic salts, carbohydrates, amino acids, buffering agents, vitamins, and compounds to simulate the natural cell environment.
Claims20
A substitute for serum in cell culture media capable of long-term culture of suspension and monolayer cells and hybridoma cells and capable of supporting fusion of cells to form hybridomas, comprising fetuin, transferrin, albumin, cholesterol and phosphatidylcholine having the general formula:
- ##STR3## wherein R.sub.1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid;
- and, R.sub.2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid;
- or
- R.sub.1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid;
- and, R.sub.2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid;
- and,
- R.sub.1 and R.sub.2 are chemically bonded to the respective carbon atoms of the phosphatidylcholine.
In cell culture media capable of long-term growth of suspension cells, monolayer cells and hybridoma cells, and capable of fostering the fusion of cells to form hybridomas, such media including salts, amino acids, carbohydrates, albumin, at least one steroid and unbound fatty acid, the improvement comprising the addition of fetuin, transferrin and phosphatidylcholine having the general formula:
- ##STR4## wherein:
- R.sub.1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid;
- and, R.sub.2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid;
- or,
- R.sub.1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid, and stearic acid;
- and, R.sub.2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, and arachidonic acid;
- and,
- R.sub.1 and R.sub.2 are chemically bonded to their respective carbon atoms of the phosphatidylcholine.
In cell culture media capable of culturing suspension cells, monolayer cells and hybridoma cells are capable of supporting fusion of cells to form hybridomas, the media including Dulbecco's Modified Eagle Medium, the improvement further including in the cell culture media fetuin, partially to fully iron saturated transferrin, and phosphatidylcholine having the general formula:
- ##STR5## wherein:
- R.sub.1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, or arachidonic acid;
- and, R.sub.2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid or stearic acid;
- or conversely,
- R.sub.1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid;
- and, R.sub.2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid;
- and,
- R.sub.1 and R.sub.2 are chemically bonded to their respective carbon atoms of the phosphatidylcholine.
An improved process for culturing suspension, monolayer and hybridoma cells and fusing cells to form hybridoma cells comprising the step of growing the cells in a culture medium comprising salts, amino acids, carbohydrates, albumin, one or more steroids, unbound fatty acids, fetuin in the range of about 9 micrograms to 1500 milligrams per liter of medium, transferrin in the range of about 3 milligrams to 300 milligrams per liter of medium and a defined phosphatidylcholine selected from the group consisting of 1-palmitoyl-2-oleoyl phosphatidylcholine and 1-oleoyl-2-palmitoyl phosphatidylcholine, wherein such defined phosphatidylcholine is in the range of about 180 micrograms to 18 milligrams per liter of medium.
Dependencies are inferred from the claim text.