# US 4,559,229 A · Avian proventriculitis vaccine

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 4,559,229 A
- Document ID: US4559229A
- Status: Granted
- Kind: A
- Application number: 06/400,024
- Filed: 1982-07-20
- Granted: 1985-12-17
- Earliest claimed: 1982-07-20
- Assignees: The Research Foundation
- Inventors: Robert K. Page; Pedro Villegas; Caswell S. Eidson; Daniel Gaudry
- Web page: https://patentgrep.com/patent/US4559229A

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 39/12: Viral antigens
- A61K 39/15: Reoviridae, e.g. calf diarrhea virus
- A61K 2039/5252: {inactivated (killed)}
- A61K 2039/54: {characterised by the route of administration}
- A61K 2039/55566: {Emulsions, e.g. Freund's adjuvant, MF59}
- C12N 2720/12034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y10S 424/816: Viral vaccine for avian species, e.g. poultry or other birds
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A process of preparing a vaccine capable of protecting poultry against avian proventriculitis which comprises: separating proventriculitis reovirus from reovirus-carrying tissue of a poultry animal diseased with proventriculitis; inoculating a cell culture capable of sustaining the growth and replication of the reovirus; extracting the virus from cellular and nonviral components, and rendering the reovirus into a form suitable for administration to poultry, yields a vaccine and an immunization method.

## Claims

### Claim 1

A vaccine capable of protecting poultry against proventriculitis, which comprises:
an antigenic material capable of inducing in said poultry an immune response against avian proventriculitis reovirus, wherein said antigenic material is selected from the group consisting of live, attenuated, and inactivated proventriculitis reovirus and immunologically active subcomponents thereof, wherein said reovirus has the identifying characteristics of ATCC No. VR2040 or a progeny thereof, and
a pharmacologically acceptable carrier.

### Claim 2 (depends on 1)

The vaccine of claim 1 wherein said carrier is a biocompatible oil.

### Claim 3 (depends on 1)

The vaccine of claim 1 wherein said reovirus is inactivated with propiolactone.

### Claim 4 (depends on 1)

The vaccine of claim 1 wherein said reovirus is attenuated by serial passage.

### Claim 5 (depends on 1)

The vaccine of claim 1 in unit dosage form.

### Claim 6 (depends on 1)

A method of protecting a poultry animal against proventriculitis which comprises:
vaccinating said poultry animal with the avian proventriculitis vaccine of claim 1, in an amount sufficient to produce an immune response against proventriculitis reovirus in said animal.

### Claim 7 (depends on 6)

The method of claim 6 wherein said vaccine comprises antigenic material selected from the group consisting of live, attenuated and inactivated proventriculitis reovirus.

### Claim 8 (depends on 6)

The method of claim 6 wherein said carrier is a biocompatible oil.

### Claim 9 (depends on 7)

The method of claim 7 wherein said reovirus is inactivated with propiolactone.

### Claim 10 (depends on 7)

The method of claim 7 wherein said reovirus is attenuated by serial passage.

### Claim 11 (depends on 6)

The method of claim 6 wherein said poultry animal is a reproductive hen.

### Claim 12

Avian proventriculitis reovirus having the identifying characteristics of ATCC No. VR2040 or a progeny thereof.