Composition, analytical element and method for the detection of bacteria
AssigneeEastman Kodak Company
InventorsRobert T. Belly, Laurie J. Clements
Life of this patent
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Abstract
A composition, an analytical element and a method for the detection of bacteria in specimen samples, e.g. biological fluids, are disclosed. The composition optionally, but preferably, comprises a metabolizable substrate (e.g. glucose) and a benzindole dye which undergoes a detectable color change when incubated in admixture with a bacterial microorganism. Useful dyes include particular benz[cd]indole, benz[e]indole and benz[g]indole compounds. The described analytical element contains this composition, preferably, in a spreading zone. Detection of bacteria can be accomplished by bringing the composition or element into contact with a specimen sample. This invention is particularly useful in detection of significant bacteriuria.
Claims25
A composition for the detection of a bacterial microorganism, said composition comprising a metabolizable substrate and a benzindole dye selected from the group consisting of benz[cd]indole dyes, benz[e]indole dyes and benzy[g]indole dyes, provided said dye undergoes a detectable color change when incubated at 37.degree. C. in admixture with an aqueous suspension of said bacterial microorganism.
An analytical element for detecting a bacterial microorganism, said element comprising an absorbent material containing a benzindole dye selected from the group consisting of benz[cd]indole dyes, benz[e]indole dyes and benzy[g]indole dyes, provided said dye undergoes a detectable color change when incubated at 37.degree. C. in admixture with an aqueous suspension of said bacterial microorganism.
An analytical element for detecting a bacterial microorganism, said element including a support having thereon and, in fluid contact, reagent and spreading zones, said reagent zone containing a benzindole dye selected from the group consisting of benz[cd]indole dyes, benz[e]indole dyes and benz[g]indole dyes, provided that said dye undergoes a detectable color change when incubated at 37.degree. C. in admixture with an aqueous suspension of said bacterial microorganism.
A method for the detection of a bacterial microorganism comprising bringing a specimen sample suspected of containing a bacterial microorganism for analysis into contact with a benzindole dye selected from the group consisting of benz[cd]indole dyes, benz[e]indole dyes and benz[g]indole dyes, provided that said dye undergoes a detectable color change when incubated at 37.degree. C. in admixture with an aqueous suspension of said bacterial microorganism and observing said color change which indicates the presence of said microorganism.
A method for the detection of a bacterial microorganism, said method comprising contacting a urine sample suspected of containing a bacterial with a benzindole dye selected from the group consisting of benz[cd]indole dyes, benz[e]indole dyes and benzy[g]indole dyes provided that said dye undergoes a detectable color change when incubated at 37.degree. C. in admixture with an aqueous suspension of said bacterial microorganism and observing said color change which indicates the presence of said microorganism.
A method for the detection of a bacterial microorganism in a specimen sample, said method comprising bringing said sample suspected of containing a bacterial microorganism into contact with an analytical element including a support and a spreading/reagent zone containing a benzindole dye selected from the group consisting of benz[cd]indole dyes, benz[e]indole dyes and benz[g]indole dyes, provided said dye undergoes a detectable color change when incubated at 37.degree. C. in admixture with an aqueous suspension of said bacterial microorganism and observing said color change which indicates the presence of said microorganism.
Dependencies are inferred from the claim text.