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US 4,546,082 AGranted
E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
AssigneeThe Regents of the Univ. of California
InventorsJanet Kurjan, Ira Herskowitz
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Abstract
A method for producing secretable and processable biologically useful polypeptides in yeast is provided utilizing vectors including at least a segment of the gene coding for S. cerevisiae alpha-factor.
Claims10
A DNA expression vector capable of expressing in yeast cells a product which is secreted from said yeast cells, said vector comprising at least a segment of alpha-factor precursor gene and at least one segment encoding a polypeptide.
A method for producing a DNA expression vector containing alpha-factor gene comprising the steps of
- (a) transforming a MAT alpha2 leu2 yeast strain with a gene bank constructed in plasmid YEp13;
- (b) selecting for Leu.sup.+ transformants from the population formed in step (a);
- (c) replating the transformants from step (b) and
- (d) screening for alpha-factor producing colonies.
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