Psoralen inactivated double-stranded RNA viral vaccines
AssigneeAdvanced Genetics Research Institute
InventorsRichard E. Giles, David R. Stevens, Gary P. Wiesehahn
Life of this patent
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Abstract
Novel vaccines of double-stranded RNA viruses are prepared by psoralen inactivation under mild conditions in an inert atmosphere, optionally in the presence of a mild singlet oxygen scavenger. The resulting inactivated virus can be used as a vaccine for inoculation of hosts to provide for the stimulation of the immune system to the virus.
Claims11
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises at least one furocoumarin-inactivated BTV serotype in from about 10.sup.6 to 10.sup.9 pfu/ml, wherein said inactivation is as a result of irradiation of BTV in the presence of an inactivating furocoumarin with long wavelength ultraviolet light at a temperature below about 40.degree. C. for a time sufficient to inactivate said BTV to a non-infectious degree, and an immunologic adjuvant.
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises BTV serotype 11 inactivated with 4'-aminomethyl-4,5',8-trimethylpsoralen by irradiation with long wavelength ultraviolet light at a temperature in the range of about -10.degree. to 25.degree. C. for a time sufficient to inactivate said BTV to become non-infectious, said BTV being present in an amount of about 10.sup.6 to 10.sup.9 pfu/ml, and an immunologic adjuvant.
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises at least one furocoumarin-inactivated BTV serotype in from about 10.sup.6 to 10.sup.9 pfu/ml, wherein said inactivation is as a result of irradiation of BTV in the presence of an inactivating furocoumarin with long wavelength ultraviolet light at a temperature below about 40.degree. C. for a time sufficient to inactivate said BTV to a non-infectious degree.
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises BTV serotype 11 inactivated with 4'-aminomethyl-4,5',8-trimethylpsoralen by temperature in the range of about -10.degree. to 25.degree. C. for a time sufficient to inactivate said BTV to become non-infectious, said BTV being present in an amount of 10.sup.6 to 10.sup.9 pfu/ml.
A method for producing a vaccine for inoculation of a mammalian host susceptible to infection by bluetongue virus (BTV), which method comprises inactivating at least one BTV serotype by exposure to long wavelength ultraviolet light in the presence of a furocoumarin at a temperature below about 40.degree. C. for a time sufficient to inactivate said BTV to a non-infectious degree, and combining said inactivated BTV with an appropriate adjuvent.
A method for producing a vaccine for inoculation of a mammalian host susceptible to infection by bluetongue virus (BTV), which method comprises exposure of at least one BTV serotype to long wavelength ultraviolet light in the presence of 4'-aminomethyl-4,5', 8-trimethylpsoralen at a temperature in the range from about -10.degree. C. to 25.degree. C. for a time sufficient to inactivate the BTV to a non-infectious degree, and combining said inactivated BTV with a suitable adjuvant.
Dependencies are inferred from the claim text.