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US 4,545,986 AGranted
Timothy grass antigen specific anti-idiotypic antibodies
AssigneeResearch Corporation
InventorArthur Malley
Life of this patent
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AssigneeResearch Corporation
InventorArthur Malley
Estimating the term…
This invention provides a number of immunological reagents useful for the suppression of allergic reactions induced by Timothy-grass pollen allergen (or cross-reacting allergens thereof) and for methods of obtaining said reagents. The reagents include: (1) an anti-idiotypic antibody (anti-IgE.sub.id) directed to the allergen (Timothy grass antigen) binding site of an IgE immunoglobulin; (2) an anti-idiotypic antibody directed to the allergenic T-cell helper factor (T.sub.HF) derived from Timothy grass pollen antigen stimulated lymphocytes; (3) unique methods for generating antigen-specific T-suppressor cells (T.sub.S); (4) a unique method for producing antigen-specific suppressor T cell factors (T.sub.SF); (5) a method for fractionation of T.sub.SF ; and (6) a specific T cell factor, T.sub.SF2. The invention further provides pharmaceutical compositions comprising the above anti-idiotypic antibodies or suppressor factor derived from T.sub.s cells.
A method of preparing an anti-idiotypic antibody directed against (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific allergic antibody, IgE, said anti-idiotypic antibody, anti-IgE.sub.id, being specifically reactive with the idiotypic determinants on said IgE and (1) antigen B- and (2) cross reactive grass antigen-specific T and B cells comprising:
The antibody anti-IgE.sub.id.
A method of preparing an anti-idiotypic antibody directed against (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific allergenic T-helper factor, T.sub.HF, said anti-idiotypic antibody, anti-T.sub.HF, being specifically reactive with the idiotypic determinants on said T.sub.HF and (1) antigen B- and (2) cross reactive grass antigen-specific T and B cells comprising:
The antibody, anti-T.sub.HF.
A method of producing Timothy grass antigen B- and crossreactive grass antigen-specific T suppressor cells, T.sub.S, comprising:
A method of producing (1) Timothy grass antigen B- and (2) cross reactive grass-antigen specific suppressor T-cell factor, T.sub.SF, by repeatedly freezing and thawing Ts cells and separating said Ts cells from T.sub.SF.
A method for producing (1) Timothy grass antigen B- and (2) cross reactive grass antigen-specific suppression T-cell factor, T.sub.SF, comprising:
A method of fractionating T.sub.SF into separate factors, T.sub.SF.sbsb.1 and T.sub.SF.sbsb.2, comprising the steps of:
The protein fraction T.sub.SF.sbsb.2.
A method of producing Timothy grass antigen B-and cross reactive grass antigen-specific T suppressor cells, T.sub.S, comprising:
Dependencies are inferred from the claim text.