Culture media from clarified diary whey lactose permeates
AssigneeIGI Biotechnology, Inc.
InventorsKathleen M. Keggins, Ann C. Davis, Edward M. Sybert, Thomas D. Mays, Robert A. Milch
Life of this patent
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Abstract
A method for separating bacteriological growth inhibitors from sweet whey ultrafiltrate to form a mother liquor which is useful as a bacteriological culture media and a precipitate which is useful as a food grade additive to cause clouding, stabilization, emulsification, and thickening of food, pharmaceutical, and cosmetic compositions.
Claims17
A dry powder microbiological culture medium which can be reconstituted with water and autoclaved to form a clear, pH neutral culture medium capable of supporting the growth of microorganisms under suitable growth conditions, said culture medium being prepared by a process consisting essentially of:
- (a) raising the pH of a dairy whey lactose permeate having a pH below about 7 to a selected pH between pH 8 and pH 10 at which essentially all of the dissolved solids which would become insoluble when the permeate is autoclaved for 10-20 minutes at 121 degrees C. and 15 psi precipitate as microcrystalline solids;
- (b) separating the supernatant from the resulting precipitate to form a microcrystalline solid phase consisting essentially of said previously dissolved solids;
- (c) removing components having a molecular weight above about 100 kdal from the supernatant to form a lactose-rich supernatant which is essentially free of said dissolved solids;
- and
- (d) drying the resultant supernatant to form said dry powder microbiological culture medium.
Dependencies are inferred from the claim text.