Human T cell lines and method of producing same
OwnerYuichi Yamamura, Tadamitsu Kishimotoinventors
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Abstract
This invention provides a human parental T cell line which can fuse with normal human T cells, and hybrid cell lines which are prepared by fusion of the human parental T cells and the human T cells, and which are capable of producing lymphokines. This invention also provides methods of obtaining such human T cell lines, and a method of producing lymphokines from such hybrid cell lines.
Claims12
A hypoxanthine-guanine-phosphoribosyl-transferase (HGPRT) deficient human T leukemic cell line which has the identifying characteristics of ATCC number CRL-8081.
A human T hybrid cell line which is formed by fusing the cell line of HGPRT deficient human T leukemic cell having the identifying characteristics of ATCC number CRL-8081 with normal human T cells.
A method of producing a lymphokine comprising, culturing a hypoxanthine-guanine-phosphoribosyl-transferase (HGPRT) deficient human T leukemic cell line having the identifying characteristics of ATCC number CR2-8081 under conditions sufficient to form lymphokine.
A method of producing a HGPRT deficient human T leukemia cell line having the identifying characteristics of ATCC number CRL-8081 comprising culturing human T leukemia cell line CCRF-CEM in a medium containing 8-azaguanine and recovering said HGPRT deficient human T leukemia cell line.
A method of producing a human T hybrid cell line comprising fusing HGPRT deficient human leukemic T cells having the identifying characteristics of ATCC number CRL-8081 with normal human T cells with use of a fusion promoter.
Dependencies are inferred from the claim text.