Loading patent…
AssigneeBoehringer Mannheim GmbH
InventorsElli Rauscher, Ulrich Neumann, August W. Wahlefeld, Alexander Hagen, Wolfgang Gruber, Joachim Ziegenhorn, Eugen Schaich, Ulfert Deneke, Gerhard Michal, Gunter Weimann
Estimating the term…
.alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided.
In a process for the determination of alpha-amylase by the enzymatic splitting of an alpha-amylase substrate and measurement of a fission product, the improvement comprising using, as a substrate, an enzymatically produced maltoheptaose compound of the formula ##STR9## in which R is a phenylglucoside, mononitrophenyl-glucoside, dinitrophenylglucoside, and wherein the sugar moiety of the R radical is substituted in the alpha-configuration,
In a process for the determination of alpha-amylase by the enzymatic splitting of an alpha-amylase substrate and measurement of a fission product, the improvement comprising using a substrate consisting essentially of an alpha substituted maltoheptaose compound of the formula ##STR10## wherein R is a phenylglucoside, mononitrophenyl-glucoside, dinitrophenylglucose, and wherein the sugar moiety of the R radical is substituted in the alpha-configuration,
Dependencies are inferred from the claim text.