Fluorescent nucleic acid stains
AssigneeAbbott Laboratories
InventorsChao-Huei J. Wang, Michael E. Jolley
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Abstract
This disclosure relates to a class of compounds which are utilized as biological stains in fluorescent microscopy. In particular, the disclosure relates to a method for detecting and identifying various structures in a biological sample which comprises treating said biological sample with a compound of the present disclosure to form a complex which emits fluorescence when irradiated with incident light. This class of compounds has been shown to be useful in detecting a variety of structures such as, for example, viruses, bacteria, yeasts, fungi, reticulocytes and cells in biological samples.
Claims18
A method of detecting nucleic acids in a biological sample which comprises contacting said biological sample with an effective amount of a dye of the formula:
- ##STR8## wherein n represents an integer having a value of 0 or 1;
- m represents an integer having a value of 1 or 2;
- R represents a member selected from the group consisting of lower alkyl, lower hydroxyalkyl, dialkylaminoalkyl, pyrrolidinoalkyl and morpholinoalkyl;
- R.sub.1 and R.sub.2 independently represent a member selected from the group consisting of hydrogen, lower alkyl and lower haloalkyl;
- R.sub.3 represents a member selected from the group consisting of hydrogen, lower alkyl, lower alkoxy and amino;
- Z represents nonmetallic atoms necessary to complete a heterocyclic nucleus selected from the group consisting of a benzothiazole nucleus, indolenine nucleus, naphthothiazole nucleus, benzoselenazole nucleus, benzoxazole nucleus, quinoline nucleus, and pyridine nucleus any of which may be substituted with lower alkyl, halo, nitro, amino and dialkylamino;
- and
- X represents an anion;
- thereby producing a complex between the dye and the nucleic acids;
- irradiating the complex with light of excitation wavelength thereby causing the complex to emit fluorescence;
- and measuring the emitted fluorescence.
A method of staining tissues and fixed cells in a biological sample which comprises contacting said biological sample with an effective amount of a dye of the formula:
- ##STR9## wherein n represents an integer having a value of 0 or 1;
- m represents an integer having a value of 1 or 2;
- R.sub.1 and R.sub.2 independently represent a member selected from the group consisting of hydrogen, lower alkyl and lower halo alkyl;
- R.sub.3 represents a member selected from the group consisting of hydrogen, lower alkyl, lower alkoxy and amino;
- Z represents nonmetallic atoms necessary to complete a pyridine nucleus;
- R represents dialkylaminoalkyl;
- and
- X represents an anion, thereby producing a dye complex, irradiating the complex with light of excitation wavelength thereby causing the complex to emit fluorescence;
- and measuring the emitted fluorescence.
A method of staining cells in suspension in a biological sample which comprises contacting said biological sample with an effective amount of a dye of the formula:
- ##STR10## wherein n represents an integer having a value of 0 or 1;
- m represents an integer having a value of 1 or 2;
- R.sub.1 and R.sub.2 independently represent a member selected from the group consisting of hydrogen, lower alkyl, and lower haloalkyl;
- R.sub.3 represents a member selected from the group consisting of hydrogen, lower alkyl, lower alkoxy and amino;
- Z represents nonmetallic atoms necessary to complete an aminopyridine nucleus;
- R represents lower alkyl;
- and
- X represents an anion;
- thereby producing a dye complex, irradiating the complex with light of excitation wavelength thereby causing the complex to emit fluorescence;
- and measuring the emitted fluorescence.
Dependencies are inferred from the claim text.