Fermentative or enzymatic production of 2-keto-L-gulonic acid
AssigneeShionogi & Co., Ltd.
InventorsTakayasu Sonoyama, Shigeo Yagi, Bunji Kageyama
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Abstract
Fermentative or enzymatic production of 2-keto-L-gulonic acid from 2,5-diketo-D-gluconic acid using living or processed mutants, being defective in metabolizing 5-keto-D-gluconic acid and incapable of producing 2-keto-D-gluconic acid. The mutant is derived from 2-keto-L-gluconic acid producing microorganisms of genus Corynebacterium. The production is carried out in the presence of nitrates and/or hydrogen donors in a preliminarily sterilized fermentation broth in which a 2,5-diketo-D-gluconic acid producing microorganism of genus Gluconobacter or Erwinia has been cultivated.
Claims24
In a process for preparing 2-keto-L-gulonic acid from 2,5-diketo-D-gluconic acid in the presence of a living or processed microorganism capable of producing 2-keto-L-gulonic acid, an improvement which comprises use of a mutant (1) substantially defective in metabolizing 5-keto-D-gluconic acid and (2) substantially incapable of producing 2-keto-D-gluconic acid.
A mutant characterized in that it is substantially defective in metabolizing 5-keto-D-gluconic acid and substantially incapable of producing 2-keto-D-gluconic acid, which has been derived from a parent, a 2-keto-L-gulonic acid producing microorganism which belongs to Coryneform Group of Bacteria.
Dependencies are inferred from the claim text.