Malate dehydrogenase method
OwnerLarry H. Bernsteininventor
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Abstract
A diagnostic method is disclosed, based on the automated kinetic determination of LD and MD isoenzymes in serum, which utilizes the comparison of two rates of LD and MD activity at different concentrations of substrate. It compares the first activity to the second concentration (activity) to obtain a ratio of activities relative to the two said activities with the first activity in the denominator and the second activity in the numerator and then determining the amount of MD activity enhancement (accelerated activity) by observing the ratio of measured MD activity.
Claims5
A method of indicating the presence of neoplastic tissue in a patient comprising:
- (a) providing a body fluid from said patient,
- said body fluid comprising cytoplasmic malate dehydrogenase,
- (b) determining whether said body fluid comprises aberrant malate dehydrogenase by determining the Kms using oxaloacetate,
- whereby the presence of aberrant malate dehydrogenase indicates the presence of neoplastic cells in said patient.
A method of indicating the presence of neoplastic tissue in a patient comprising:
- (a) providing a body fluid from said patient, said body fluid comprising cytoplasmic malate dehydrogenase,
- (b) determining whether said body fluid comprises aberrant malate dehydrogenase, said determining comprising reacting oxaloacetate using said cytoplasmic malate dehydrogenase,
- whereby the presence of aberrant malate dehydrogenase indicates the presence of neoplastic cells in said patient.
A method of indicating the presence of neoplastic tissue in a patient comprising:
- (a) providing body fluid from said patient, said body fluid comprising cytoplasmic malate dehydrogenase,
- (b) determining whether said body fluid comprises aberrant malate dehydrogenase,
- said determining comprising a reaction using said cytoplasmic malate dehydrogenase,
- whereby the presence of aberrant malate dehydrogenase indicates the presence of neoplastic cells in said patient.
Dependencies are inferred from the claim text.