Stabilization of oxidase
AssigneeWako Pure Chemical Industries, Ltd.
InventorsYoshinobu Miyashita, Shinji Satomura
Life of this patent
Estimating the term…
Abstract
An oxidase selected from the group consisting of glycerol-3-phosphate oxidase, choline oxidase and glucose oxidase can be stabilized by adding thereto an acidic amino acid or a salt thereof. The resulting stabilized composition can be used for quantitatively determining the content of glycerol-3-phosphate, choline, triglyceride, glucose, etc., in a biological fluid.
Claims28
A process for stabilizing an oxidase which comprises adding an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight to an aqueous solution of an oxidase selected from the group consisting of glycerol-3-phosphate oxidase, and choline oxidase.
A process for quantitatively determining the glycerol-3-phosphate content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing
- glycerol-3-phosphate oxidase in an amount of 1 to 20 units/ml,
- a buffer solution in an amount sufficient to maintain the pH near neutral value,
- an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight based on the weight of the aqueous solution containing the oxidase,
- peroxidase, 4-aminoantipyrine, and an oxidizable color producing indicator in amounts sufficient to produce a color,
- and measuring the color produced colorimetrically.
A process for quantitatively determining the triglyceride content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing
- glycerol-3-phosphate oxidase in an amount of 1 to 20 units/ml,
- a buffer solution in an amount sufficient to maintain the pH near neutral value,
- an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight of the aqueous solution,
- lipase, glycerol kinase, peroxidase, 4-aminoantipyrine and an oxidizable color producing indicator in amounts sufficient to produce a color,
- and measuring the color produced colorimetrically.
A process for quantitatively determining the choline content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing
- choline oxidase in an amount of 1 to 20 units/ml,
- a buffer solution in an amount sufficient to maintain the pH near neutral value,
- an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight, based upon the weight of the aqueous solution,
- peroxidase, 4-aminoantipyrine and an oxidizable color producing indicator in sufficient amounts to produce a color, and
- measuring the color produced colormetrically.
A process for measuring the activity of choline esterase in a biological fluid which comprises adding to the biological fluid an aqueous solution containing
- choline oxidase in an amount of 1 to 20 units/ml,
- a buffer solution in an amount of 1 to 5% by weight based on the weight of the solution,
- peroxidase, choline benzoyl chloride, 4-aminoantipyrine and an oxidizable color producing indicator in amounts sufficient to produce a color,
- adding to the resulting mixture a reaction stopper solution, and
- measuring the color produced colorimetrically.
A stabilized composition comprising
- an oxidase selected from the group consisting of glycerol-3-phosphate oxidase, and choline oxidase,
- an acidic amino acid or a salt thereof in an amount equal to 1 to 5% by weight of the composition, and
- a buffer solution in an amount sufficient to maintain the pH near neutral value.
Dependencies are inferred from the claim text.