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US 4,542,608 AGranted
Mushroom cultivation
AssigneeEverbloom Mushrooms
InventorKok K. Tan
Life of this patent
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Abstract
A process for the manufacture of mushroom spawn comprises swelling cereal grains in water, sterilizing the swollen grain, e.g. in admixture with calcium carbonate, inoculating the sterile grain with a culture, e.g. in the form of a homogenate, and incubating the inoculated grains. The use of, say, CaCO.sub.3 prevents grain aggregation.
Claims4
A process for the manufacture of mushroom spawn, which comprises the steps of:
- (1) soaking cereral grains in water at 0-10 degrees C. (32-50 degrees F.) for a time sufficient to obtain swollen grains;
- (2) draining off any excess water;
- (3) heat-sterilizing said swollen grains in a first heat-sterilization stage, under conditions such that said grains are substantially prevented from aggregation;
- (4) after an interval, in which spores not killed in the previous heat-sterilization stage are allowed to germinate, heat-sterilizing said swollen grains in a further heat-sterilization stage, again under conditions such that said grains are substantially prevented from aggregation;
- (5) if desired, repeating step (4);
- (6) inoculating the thus-heat-sterilized grains with a mushroom cell culture;
- and
- (7) incubating the thus-inoculated grains, thereby obtaining muchroom spawn.
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Dependencies are inferred from the claim text.