Polyacrylamide cross-linked with a polysaccharide resin as electrophoretic gel medium
AssigneeFMC Corporation
InventorSamuel Nochumson
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Abstract
An electrophoresis medium, comprising a copolymer of acrylamide and an ethylenically unsaturated resin formed by replacing at least some of the hydroxyl hydrogens in a polysaccharide with an ethylenically unsaturated group, is described.
Claims2
A polyacrylamide film, bearing an electrophoretic pattern, said film comprising polyacrylamide cross-linked with a polysaccharide resin having a weight average molecular weight of from about 5000 to about 10.sup.6 daltons and wherein there has been substituted for at least some of the hydroxyl hydrogens in said resin an ethylenically unsaturated group of from 2 to 12 carbon atoms capable of cross-linking with acrylamide in the presence of a catalyst to form said cross-linked polyacrylamide.
A method of preparing a polyacrylamide film bearing an electrophoretic pattern comprising carrying out an electrophoretic separation in an electrophoretic gel comprising polyacrylamide cross-linked with a polysaccharide resin having a weight average molecular weight of from about 5000 to about 10.sup.6 daltons and wherein there has been substituted for at least some of the hydroxyl hydrogens in said resin an ethylenically unsaturated group of from 2 to 12 carbon atoms capable of cross-linking with acrylamide in the presence of a catalyst to form said cross-linked polyacrylamide and drying the gel to form the film.
Dependencies are inferred from the claim text.