Back to results
US 4,541,952 AGranted
Purification method of human interferon
AssigneeToray Industries, Inc.
InventorsKazuo Hosoi, Hitoshi Ozawa
Life of this patent
Estimating the term…
Abstract
A method for the purification of human interferon by the successive treatments of a crude interferon solution with an immobilized carrier which is coupled with a blue dye and a chelating carrier which contains a chelating residue chelated with at least one metal ion selected from the group consisting of Co.sup.2+, Ni.sup.2+ and Zn.sup.2+.
Claims22
A method of purifying human beta-interferon, comprising the successive steps of:
- (a) adsorbing a crude beta-human interferon solution on an immobilized blue carrier,
- (b) contacting the immobilized blue carrier with an eluant to produce an eluate containing the interferon,
- (c) contacting said eluate with a carrier having a chelating residue and including at least one metal ion selected from the group consisting of Co.sup.2+, Ni.sup.2+ and Zn.sup.2+, wherein said metal ion is chelated to the chelating residue so a to absorb the interferon, and
- (d) contacting said carrier with an eluant to produce a purified and concentrated interferon solution.
1 more element
Dependencies are inferred from the claim text.