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US 4,540,664 AGranted
Method of saccharifying cellulose
AssigneeThe United States of America as represented by the United States Department of Energy
InventorsEric A. Johnson, Arnold L. Demain, Ashwin Madia
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Abstract
A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.
Claims16
A method of saccharifying cellulose comprising:
- incubating said cellulose in an incubation mixture with a substantially cell-free cellulase-containing broth from Clostridium thermocellum and a quantity of a reducing agent sufficient to allow saccharification.
Dependencies are inferred from the claim text.