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AssigneePierrel S.p.A.
InventorsLuciano Toscano, Leonard M. Cappelletti
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From the fermentation carried, out with mutants blocked in the synthesis respectively of erythromycin and of oleandomycin, namely Streptomyces erythreus ATCC 31772 and Streptomyces antibioticus ATCC 31771, using as the substrate a derivative of erythronolide A, namely (8S)-B-fluoroerythronolide A, a derivative of erythronolide B, namely (8S)-B-fluoroerythronolide B, or a derivative of 3-O-mycarosyl-erythronolide B, namely 3-O-mycarosyl-(8S)-fluoroerythronolide B, the corresponding (8S)-8-fluoro derivatives of the erythromycins A,B, C and D, as well as 3-O-oleandrosyl-5-desosaminyl-(8S)-8-fluoroerythronolide A and 3-O-oleandrosyl-5-O-desosaminyl-(8S)-8-fluoroerythronolide B, all belonging to the class of the marcolide antibiotics are obtained. The preparation of the aforesaid substrate comprises the convention of erythronolide A, erythronolide B or 3-O-mycarosyl-erythronolide B into the corresponding hemiacetal, the reaction of the latter with a compound capable of generating electrophlic fluorine and the opening of the resulting acetal with aqueous acid.
A biologically pure culture of Streptomyces erythreus ATCC 31772, useful for microbiological processes for the preparation of antibiotics and blocked in the erythromycin synthesis.
A process for the preparation of Streptomyces erythreus ATCC 31772, characterized in that a stock of Streptomyces erythreus producer of erythromycin is subjected to mutagenesis such as to kill at least 99% of the spores, and the surviving spores are analized and selected on the basis of their capacity of recognizing and converting (8S)-8-fluoroerythronolide B as the substrate in a fermentation process.
A process for the production of a macrolide antibiotic selected from the group consisting of:
Dependencies are inferred from the claim text.