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US 4,539,204 AGranted
Gelatin based synthetic blood and a method of making the same
AssigneeNeomed, Inc.
InventorsCharles S. Ecanow, Bernard Ecanow
Life of this patent
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AssigneeNeomed, Inc.
InventorsCharles S. Ecanow, Bernard Ecanow
Estimating the term…
A composition of matter which comprises a synthetic whole blood useful as a replacement for whole mammalian blood and a method of making the same are disclosed. The method of manufacture makes use of two gelatins, two modified fluid gelatins, or one gelatin and one modified fluid gelatin of differing isoelectric points, and selected additives. The invention also contemplates the use of only one gelatin or only one modified fluid gelatin, plus lecithin. The invention also contemplates using stroma free hemoglobin, microencapsulated stroma free hemoglobin, or synthetic liposomes containing stroma free hemoglobin incorporated in either the coacervate phase or the coacervate system of this invention. The method yields a two phase liquid aqueous system which successfully duplicates the two phase heterogeneous physico-chemical system of naturally occurring whole blood. The disclosed composition possesses many of the physiological capabilities of whole human blood.
A synthetic whole blood substitute comprising two gelatins, two modified fluid gelatins, or one gelatin and one modified fluid gelatin, having different isoelectric points, water, and sufficient alkaline substance to achieve a basic pH, said synthetic whole blood being a substantially non-polar coacervate phase.
A synthetic whole blood comprising two gelatins, two modified fluid gelatins or one gelatin and one modified fluid gelatin, having different isoelectric points, water, and sufficient alkaline substance to achieve a basic pH, said synthetic whole blood being a two-phase system, said phases being a substantially nonpolar coacervate phase and a substantially polar equilibrium water phase.
A method of making a synthetic whole blood, said method comprising (a) combining water and two gelatins, two modified fluid geltins, or one gelatin and one modified fluid gelatin, with different isoelectric points, (b) storing the combination at 15.degree.-40.degree. C., for 12-72 hours whereby said combination separates into two layers, said lower layer being a substantially non-polar coacervate phase, and said upper layer being an equilibrium water phase, (c) separating said lower phase from said upper phase and (d) adjusting the pH of said lower phase to the range of from 7.2-7.6.
A method to make a synthetic whole blood, said method comprising (a) combining water and two gelatins, two modified fluid gelatins, or one gelatin and one modified fluid gelatin, with different isoelectric points, (b) storing the combination at 15.degree.-40.degree. C., for 12-72 hours, whereby said combination separates into two layers, said lower layer being a substantially nonpolar coacervate phase, and said upper layer being a substantially polar equilibrium water phase, (c) separating said lower phase from said upper phase, (d) adjusting the pH of said lower phase to the range of 7.2-7.6 and (e) combining said lower coacervate phase of step (c) with said previously separated upper equilibrium water phase, thereby forming a two-phase system.
Dependencies are inferred from the claim text.